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Promega mumlv-rt reverse transcriptase 5× buffer
Mumlv Rt Reverse Transcriptase 5× Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5%C3%97+reverse+transcriptase+(rt)+buffer/reverse+transcriptase+enzyme/pmc02453129-358-53-27
Average 90 stars, based on 1 article reviews
mumlv-rt reverse transcriptase 5× buffer - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Anti-Photoaging Effects of Angelica acutiloba Root Ethanol Extract in Human Dermal Fibroblasts
Article Snippet: Total RNA from human dermal fibroblasts was extracted with 1 mL of TRIzol (Invitrogen) per the manufacturer instructions, and 5 μg samples were reverse transcribed in 40-μL of reaction mixture containing 8 μL 5× M-MLV RT (Murine Leukemia Virus Reverse Transcriptase) buffer, 3 μL dNTPs (10 mM), 0.45 μL RNasein (40 U/μL), 0.3 μL M-MLV reverse transcriptase (200 U/μL; Promega, Madison, WI, USA), and 1.5 μL of oligo dT (50 pM/μL; Bioneer, Daejeon, Korea).

Article Title: Anti-Inflammatory and Antioxidant Properties of Ethanol Extracts of Raw, Blanched, Steamed, and Sous-Vide Cooked Okra (Abelmoschus esculentus L.) in LPS or H2O2-Treated RAW264.7 Cells
Article Snippet: Briefly, equal amounts of total RNA (5 μg) were reverse transcribed in a 40 μL reaction mixture containing 8 μL Molony Murine Leu-kemia Virus Reverse Transcriptase (M MLV RT) 5× buffer, 3 μL 10 mM dNTPs, 0.45 μL 40 U/μL RNase inhibitor, 0.3 μL 200 U/μL M MLV RT (Promega Corporation) and 3.75 μL 20 μM oligo dT (Bioneer Corporation, Oakland, CA, USA).

Article Title: Orchid Propagation: From Laboratories to Greenhouses—Methods and Protocols
Article Snippet: Add RT mixture (19 μL comprising 7.4 μL H2O, 6 μL 5× RT buffer, 5 μL 2.5 mM dNTP, 0.5 μL M-MLV reverse transcriptase (Promega), 0.1 μL RNase inhibitor (Takara)) and then incubate at 42 °C for 70 min. 3.

Article Title: Phosphatidylcholine causes adipocyte-specific lipolysis and apoptosis in adipose and muscle tissues
Article Snippet: RNAs were transcribed to cDNA at 40°C for 60 min in a 20-μL cocktail containing 5× reverse transcriptase (RT) buffer, 15 mM dNTPs (300 U), Maloney murine leukemia virus reverse transcriptase (MMLV-RT) (Promega, Madison, WI, USA) and 100 pmol oligo-dT primer. cDNA concentration was estimated by the quantitative RT-PCR method using 2× iQTM SYBR Green Supermix (Bio-Rad, Hercules, CA, USA), to measure the mRNA levels of each gene.

Article Title: MUC21 induces the viability and migration of glioblastoma via the STAT3/AKT pathway
Article Snippet: Total RNA was reverse transcribed into cDNA at 42 ̊C for 1 h using M-MLV reverse transcriptase (cat. no. M1701; Promega Corporation, includes M-MLV 5X Reaction Buffer 5 μl, dNTP, 10 mM 1.25 μl, Recombinant RNasin ® Ribonuclease Inhibitor 25 units, M-MLV RT 200 units and Nuclease-Free Water to final volume of 25 μl). qPCR was then performed using the SYBR Ex Taq kit (cat. no. 638319; Takara Bio, Inc.) and used according to the manufacturer's protocol.

Article Title: Antioxidant Activity and Anti-wrinkle Effects of Aceriphyllum rossii Leaf Ethanol Extract
Article Snippet: The total RNA from the human dermal fibroblasts was extracted with 1 mL of TRIzol (Invitrogen, CA) per the manufacturer instructions, and 5 μg samples were reversetranscribed in 40-μL of a reaction mixture containing 8 μL of 5 × Molony murine leukemia virus reverse transcriptase (M-MLV RT) buffer, 3 μL of dNTPs (10 mM), 0.45 μL of RNasein (40 U/μL), 0.3 μL of M-MLV reverse transcriptase (200 U/μL; Promega, USA), and 1.5 μL of oligo dT (50 pM/μL; Bioneer, Korea).

Article Title: Gene expression of NMDA and AMPA receptors in different facial motor neurons.
Article Snippet: Objectives/Hypothesis: Facial motor neurons (FMNs) are involved in the remodeling of the facial nucleus in response to peripheral injury.. This study aimed to examine the gene expression of alpha-amino-3-hydroxy-5-methylisoxazole-4propionic acid receptor (AMPAR) and N-methyl-D-aspartate subtype of ionotropic glutamate receptor (NMDAR) in reinnervating dormant FMNs after facial nerve axotomy.. Study Design: Animal study.

Reverse Transcription:

Article Title: Antioxidant Activity and Anti-wrinkle Effects of Aceriphyllum rossii Leaf Ethanol Extract
Article Snippet: .. The total RNA from the human dermal fibroblasts was extracted with 1 mL of TRIzol (Invitrogen, CA) per the manufacturer instructions, and 5 μg samples were reverse-transcribed in 40-μL of a reaction mixture containing 8 μL of 5 × Molony murine leukemia virus reverse transcriptase (M-MLV RT) buffer, 3 μL of dNTPs (10 mM), 0.45 μL of RNasein (40 U/μL), 0.3 μL of M-MLV reverse transcriptase (200 U/μL; Promega, USA), and 1.5 μL of oligo dT (50 pM/μL; Bioneer, Korea). .. Single-stranded cDNA was amplified by PCR using 4 μL of 5 × green Go Taq Flexi buffer, 0.4 μL of dNTPs (10 mM), 0.1 μL Taq polymerase (5 U/μL), 1.2 μL of MgCl 2 (25 mM; Promega, USA), and 0.4 μL each of (20 pM/μL) specific sense and anti-sense primers for MMP-1 or β-actin.

Virus:

Article Title: Antioxidant Activity and Anti-wrinkle Effects of Aceriphyllum rossii Leaf Ethanol Extract
Article Snippet: .. The total RNA from the human dermal fibroblasts was extracted with 1 mL of TRIzol (Invitrogen, CA) per the manufacturer instructions, and 5 μg samples were reverse-transcribed in 40-μL of a reaction mixture containing 8 μL of 5 × Molony murine leukemia virus reverse transcriptase (M-MLV RT) buffer, 3 μL of dNTPs (10 mM), 0.45 μL of RNasein (40 U/μL), 0.3 μL of M-MLV reverse transcriptase (200 U/μL; Promega, USA), and 1.5 μL of oligo dT (50 pM/μL; Bioneer, Korea). .. Single-stranded cDNA was amplified by PCR using 4 μL of 5 × green Go Taq Flexi buffer, 0.4 μL of dNTPs (10 mM), 0.1 μL Taq polymerase (5 U/μL), 1.2 μL of MgCl 2 (25 mM; Promega, USA), and 0.4 μL each of (20 pM/μL) specific sense and anti-sense primers for MMP-1 or β-actin.



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Journal: iScience

Article Title: Time course transcriptomic profiling suggests Crp/Fnr transcriptional regulation of nosZ gene in a N 2 O-reducing thermophile

doi: 10.1016/j.isci.2024.111074

Figure Lengend Snippet:

Article Snippet: Maxima H Minus Reverse Transcriptase (200U/μL) with 5×RT Buffer , ThermoFisher , Cat# EP0751.

Techniques: Virus, Recombinant, Reverse Transcription, Isolation, Control, Sequencing, Mass Spectrometry, Software, In Silico, Targeted Proteomics